Juliette Trullemans began her artistic career with professor Hendrickx in Brussels (Institut Bischoffsheim). She was particularly gifted and decided therefore to specialise in flower-painting in the workshop of Capeininck in Ghent. There she studied very closely the Romantic botanic iconography and spent hours with the precise rendering of floral motives.
At the same time she was feeling the need to explore the nature outside the atelier. With Rodolphe Wytsman, whom she met in the workshop of Capeininck, she shared the same interest and admiration for the plein-air painting of the Impressionists like Monet and Pissarro. Wytsman, being a founding member of the Groupe des XX, introduced her to this circle of the Belgian avant-garde artists. The modern spirit of the group motivated her to pursue painting outside the atelier. She concentrated on the fugitive impressions of light and shade reflecting on the vegetation around her.
Juliette and Rodolphe, who married in 1886, enjoyed painting together at the picturesque sites in the outskirts of Brussels: La Hulpe or in the woods of Soignies. At that time they became friends with the writer Camille Lemonnier, whose garden Juliette used to paint. In this period Juliette proved her special qualities as a highly sensitive landscape painter. In the house of Lemonnier she used to meet the Belgian Impressionist – founder of the Luminist movement - Emile Claus.
In 1892 the couple bought a house in Linkebeek at the outskirts of Brussels. The country house possessed a beautiful garden, where Juliette Wytsman-Trullemans used to pick her motives. Her most impressive paintings date back in this period of her life.
With the outbreak of the First World War the couple was forced to leave the country. They settled in Rotterdam, where Rodolphe organised meetings and exhibitions of his exiled fellow Belgian painters. In Dutch villages like Overschie, Bergplaats, Oisterwyk and Mook they have found motives that resembled closely the nature back home in Linkebeek, with its moor landscapes and the pine tree woods.
As the war ended, the couple moved back to Linkebeek. Wytsman Trullemans stayed artistically active and was exposing at the official salons until her death in 1925